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Key Documents

SRP2120

Sigma-Aldrich

RNA Polymerase II Peptide

≥70% (SDS-PAGE), human recombinant, expressed in E. coli, GST tagged

Synonyme(s) :

POLRA, RPB1, RPO2, RPOL2, hRPB220

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.26

product name

RNA Polymerase II, C-terminal domain, GST tagged human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥70% (SDS-PAGE)

Forme

frozen liquid

Poids mol.

~68.1 kDa

Conditionnement

pkg of 10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Couleur

clear colorless

Numéro d'accès NCBI

NM_000937

Numéro d'accès UniProt

P24928

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... POLR2A(5430)

Catégories apparentées

Actions biochimiques/physiologiques

The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA pol II in vivo: IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongation complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF. The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing.
The CTD works as a binding scaffold for nuclear factors through its phosphorylation. It is suggested to be also involved in chromatin structure modification, DNA damage/repair, protein degradation and synthesis, RNA degradation, snRNA (small nuclear RNA) modification, and snoRNP (small nucleolar ribonucleoprotein) biogenesis.

Forme physique

Clear and colorless frozen liquid solution

Notes préparatoires

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

RNA polymerase II C-terminal domain required for enhancer-driven transcription.
Gerber HP, et al.
Nature, 374, 660-662 (1995)
Phosphorylation and functions of the RNA polymerase II CTD.
Phatnani HP and Greenleaf AL
Genes & Development, 20, 2922-2936 (2006)
T O'Brien et al.
Nature, 370(6484), 75-77 (1994-07-07)
The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II is essential in vivo, and is found in either an unphosphorylated (IIa) or hyperphosphorylated (IIo) form. The Drosophila uninduced hsp70 and hsp26 genes, and the constitutively expressed beta-1
H Lu et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(22), 10004-10008 (1991-11-15)
The two forms of RNA polymerase II that exist in vivo, phosphorylated (IIO) and nonphosphorylated (IIA), were purified to apparent homogeneity from HeLa cells. The nonphosphorylated form preferentially binds to the preinitiation complex. RNA polymerase II in the complex was
The role of multisite phosphorylation in the regulation of RNA polymerase II activity.
M E Dahmus
Progress in nucleic acid research and molecular biology, 48, 143-179 (1994-01-01)
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