Accéder au contenu
MilliporeSigma
Toutes les photos(3)

Key Documents

SRE0115

Sigma-Aldrich

Salt Active Nuclease (SAN)

Heat-labile, recombinant, expressed in Pichia pastoris, Triton-free

Synonyme(s) :

Heat-labile salt active nuclease, endonuclease, salt active endonuclease, salt tolerant nuclease

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Produit recombinant

expressed in Pichia pastoris

Niveau de qualité

Activité spécifique

≥175 000 U/mg

Caractéristiques

Heat-labile

Composition

Triton-free

Conditionnement

pkg of 25 kU

Conditions d'expédition

dry ice

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Nucleic acids, and especially genomic DNA, often pose a problem in purification of DNA-binding proteins as they interfere with purification, downstream analysis or applications. As most nucleases are inhibited by high concentrations of salt, removal of DNA is difficult, since NaCl is used to dissociate DNA from proteins.
This Salt Active Nuclease (SAN) is a nonspecific endonuclease recombinantly produced in Pichia pastoris, with optimum activity at high salt concentrations. It is active in a variety of buffers and can be easily inactivated by treatment with a reducing agent. These features make it particularly useful in the purification of proteins and removal of DNA and RNA from molecular biology reagents. It can be used on cell lysates for viscosity removal, and additionally at the elution step of purification, where there are smaller volumes and a purer protein - allowing for effective and efficient DNA removal.
This Salt Active Nuclease (SAN) is a heat-labile nuclease that has optimal activity at high salt concentrations and is useful during protein purification.

Application

The high salt tolerance and easy removal makes this SAN useful in protein purification schemes, especially in combination with IMAC (Immobilized metal affinity chromatography) media used for purification of poly-His-tagged proteins.

Caractéristiques et avantages

  • Active at up to 1M NaCl
    Tolerates imidazole and can be separated from most proteins by cation exchange or inactivated by reducing agents.

Définition de l'unité

One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA (D1501, Sigma) in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25°C), 5 mM MgCl2, 500 mM NaCl.

Reconstitution

In solution

Autres remarques

Temp: 5 – 40°C, optimal: ~ 35°C
Salt concentration (NaCI / KCI): 150 - 900 mM, optimal: 400 - 650 mM
Mg2+: >1 mM is required for activity, optimal: 5 - 50 mM
pH: 7.3 – 10, optimal: 8.2 - 9.2
Not recommended to use in presence of SDS or urea.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique