SAB5801046

Alpaca Anti-Human IgG(Fcγ) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonyme(s) : Recombinant Alpaca single variable domain secondary Nanobody
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: alpaca
• Produit recombinant: expressed in E. coli
• Conjugué: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• Forme d'anticorps: affinity purified immunoglobulin
• Type de produit anticorps: secondary antibodies
• Clone: recombinant monoclonal
• Forme: lyophilized solid
• Poids mol.: 14 kDa
• Espèces réactives: Alpaca, human
• Technique(s): ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• Taille: 0.1 mL
• Couleur: colorless
• Isotype: VHH
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Alpaca Anti-Human IgG(Fcγ) single domain Antibody is designed to specifically detect human IgG Fcγ fragment. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Human IgG(Fcγ) single domain Antibody react with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG.
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Spécificité

Human IgG(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogène

Recombinant Alpaca single variable domain secondary nano antibody

Conditionnement

100 μg in Glass bottle

Forme physique

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Stockage et stabilité

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Autres remarques

Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317,H412
• Conseils de prudence: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Classification des risques: Aquatic Chronic 3 - Skin Sens. 1
• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable