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Key Documents

23-035-M

Sigma-Aldrich

UbcH5c Protein, active, 10 µg

Active, full length, recombinant human UbcH5c. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

Synonyme(s) :

Ubiquitin-conjugating enzyme E2 D3, Ubiquitin-protein ligase D3, Ubiquitin-conjugating enzyme E2-17 kDa 3, UBE2D3

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.41

Source biologique

human

Niveau de qualité

100
300

Fabricant/nom de marque

Upstate®

Technique(s)

activity assay: suitable

Numéro d'accès NCBI

NM_181888

Description générale

N-terminal HA, 6His-tagged, recombinant human UbcH5c full length, expressed in E.coli.
Ubiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate. The UbcH5 family (UbcH5a, UbcH5b and UbcH5c) have been shown to be the most active class of E2 enzymes in cell extracts and are associated with the control of a number of proteins including the transcriptional regulators p53, IκBα and HIF-1α.

Application

Ubiquitination Cascade Comoponent: E2

Forme physique

Purified using immobilized metal affinity chromatography.

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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