SAB5801018

Alpaca Anti-Mouse IgM(µ chain) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonyme(s) : Recombinant Alpaca single variable domain secondary Nanobody
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: alpaca
• Produit recombinant: expressed in E. coli
• Conjugué: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• Forme d'anticorps: affinity purified immunoglobulin
• Type de produit anticorps: secondary antibodies
• Clone: recombinant monoclonal
• Forme: lyophilized solid
• Poids mol.: 14 kDa
• Espèces réactives: Alpaca, mouse
• Technique(s): ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• Taille: 0.1 mL
• Couleur: colorless
• Isotype: VHH
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Alpaca Anti-Mouse IgM(µ chain) single domain Antibody is designed to specifically detect mouse IgM. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgM(µ chain) single domain Antibody reacts with the µ chain of mouse IgM selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in the primary immune response. Unlike IgG, IgM does not contain a hinge region but does contain an additional constant domain. The monomeric form IgM has a molecular weight of 180 KD. It is classically represented as a pentamer of the basic four chain structure held together by a J chain but can also exist in a hexameric form without the J chain and as a monomer on the surface of B-cells.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Spécificité

Fc region of mouse IgM (µ chain specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogène

Recombinant Alpaca single variable domain secondary nano antibody

Conditionnement

100 μg in Glass bottle

Forme physique

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Stockage et stabilité

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Autres remarques

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317,H412
• Conseils de prudence: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Classification des risques: Aquatic Chronic 3 - Skin Sens. 1
• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable