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Key Documents

A0168

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody

Synonyme(s) :

Goat Anti-Mouse IgG (Fc specific)–HRP

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Conjugué

peroxidase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

mouse

Technique(s)

direct ELISA: 1:50,000
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Antibody is isolated from goat anti-mouse IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the Fc fragment of mouse IgG. Anti-Mouse IgG is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.

Immunogène

purified mouse IgG Fc fragment

Application

Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in
  • western blot
  • enzyme linked immunosorbent assay (ELISA) and
  • indirect immunoperoxidase assay (IIPA)

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

Forme physique

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Notes préparatoires

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Surrogate Tumor Antigen Vaccination Induces Tumor-Specific Immunity and the Rejection of Spontaneous Metastases
Lewis, J. et al
Cancer Research, 51, 169-176 (2005)
Pre-clinical development of gene modification of haematopoietic stem cells with chimeric antigen receptors for cancer immunotherapy
Larson SM, et al.
Human Vaccines & Immunotherapeutics, 13(5), 1094-1104 (2017)
Human antibodies targeting CD30+ lymphomas
Wezler X, et al.
Human Antibodies, 21(1-2), 13-28 (2012)
Structural insights of proteins in sub-cellular compartments: In-mitochondria NMR
Barbieri L, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1843(11), 2492-2496 (2014)
Identification of peptidases in Nicotiana tabacum leaf intercellular fluid
Delannoy M, et al.
Proteomics, 8(11), 2285-2298 (2008)

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