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SAB4200782

Sigma-Aldrich

Anti-Tenascin antibody, Mouse monoclonal

clone BC-24, purified from hybridoma cell culture

Synonyme(s) :

Anti-Cytotactin, Anti-GMEM, Anti-GP 150-225, Anti-Glioma-associated-extracellular matrix antigen, Anti-Hexabrachion, Anti-JI, Anti-Myotendinous antigen, Anti-Neuronectin, Anti-TN, Anti-Tenascin-C (TN-C)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

BC-24, monoclonal

Forme

buffered aqueous solution

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

ELISA: suitable
flow cytometry: suitable
immunoblotting: suitable
immunohistochemistry: 5-10 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tonsil sections

Isotype

IgG1

Numéro d'accès UniProt

P24821

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TNC(3371)

Catégories apparentées

Description générale

Monoclonal Anti-Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human tenascin. Tenascin-C (TN-C) is also known as Hexabrachion, Cytotactin, Neuronectin (NEC1). It is a high molecular mass extracellular matrix glycoprotein. Human tenascin is a disulfide-linked hexamer composed of 3 subunits of 190, 200, and 220 kDa.

Immunogène

Human tenascin

Application

Anti-Tenascin antibody has been used:
  • in immunoblotting
  • in Immunohistochemistry
  • in flow cytometry
  • in enzyme linked immunosorbent assay(ELISA)
  • for blocking tenascin-C proliferating activity

Actions biochimiques/physiologiques

Tenascin-C (TN-C) functions in cell adhesion, fibroblast migration, and other processes related to tissue remodeling and wound healing. It has been proposed that actively growing, migrating and differentiating epithelial sheets can produce factors such as (Transforming growth factor beta) TGF-β to stimulate tenascin expression. Neo-expression or increased expression of tenascin has been found in the stroma of various tumors and during normal tissue repair. Intracytoplasmic tenascin immunoreactivity has been detected in malignant melanomas and in lung carcinomas, and it serves as a marker of stromal element proliferation in invasive breast carcinomas. High-molecular mass tenascin isoform plays a role in generating a permissive environment for proliferation, invasion, and metastasis of neoplastic epithelial cells. Human tenascin contains an Arg-Gly-Asp (RGD) sequence which may function in cell adhesion and mediates cell attachment through an RGD-dependent integrin receptor.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Autres remarques

This product is for R&D use only, not for drug, household, or other uses.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

The role of tenascin-C in tissue injury and tumorigenesis
Midwood K S, et al.
Journal of Cell Communication and Signaling, 3(3-4), 287-310 (2009)
Tenascin-C modulates tumor stroma and monocyte/macrophage recruitment but not tumor growth or metastasis in a mouse strain with spontaneous mammary cancer
Talts J F, et al.
Journal of Cell Science, 112(12), 1855-1864 (1999)
Christopher R Silvers et al.
British journal of cancer, 125(10), 1399-1407 (2021-09-27)
Markers of stromal activation at future metastatic sites may have prognostic value and may allow clinicians to identify and abolish the pre-metastatic niche to prevent metastasis. In this study, we evaluate tenascin-C as a marker of pre-metastatic niche formation in
L Borsi et al.
International journal of cancer, 52(5), 688-692 (1992-11-11)
Functionally different tenascin (TN) isoforms, containing varying numbers of a 91 amino-acid motif resembling the fibronectin type-III homology repeat, may be generated by alternative splicing of the TN primary transcript. In fact, only the TN isoform containing the alternatively spliced
Gregory S Schultz et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 17(2), 153-162 (2009-03-27)
Dynamic interactions between growth factors and extracellular matrix (ECM) are integral to wound healing. These interactions take several forms that may be categorized as direct or indirect. The ECM can directly bind to and release certain growth factors (e.g., heparan
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