SAB4200738
Anti-FITC antibody, Mouse monoclonal
clone FL-D6, purified from hybridoma cell culture
Synonyme(s) :
Anti-Fluorescein isothiocyanate
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
FL-D6, monoclonal
Forme
buffered aqueous solution
Concentration
~1.0 mg/mL
Technique(s)
immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC
immunohistochemistry: suitable
indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC
Isotype
IgG1
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Description générale
Anti-FITC antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the FL-D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with FITC (fluorescein isothiocyanate)-BSA conjugate. This antibody specifically recognizes both free and conjugated FITC.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm).
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm).
Spécificité
Monoclonal Anti-FITC antibody specifically recognizes both free and conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).
Immunogène
FITC-BSA conjugate
Application
Anti-FITC antibody, Mouse monoclonal may be used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
Actions biochimiques/physiologiques
FITC (fluorescein isothiocyanate) is a commonly used protein marker including antibodies in immunofluorescent techniques. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Discard working dilution samples if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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J J Haaijman et al.
Histochemistry, 84(4-6), 363-370 (1986-01-01)
Fluorescein (Fl) and tetramethyl rhodamine (Rh) were evaluated as possible candidates for a double hapten sandwich system in enzyme immunohistology. Monoclonal antibodies were raised against Fl and Rh. Their fine-specificity was tested with a competition-like assay. A pair of Mab's
Steven J Metallo et al.
Journal of the American Chemical Society, 125(15), 4534-4540 (2003-04-10)
This paper describes the synthesis of bifunctional polyacrylamides containing pendant vancomycin (Van) and fluorescein groups, and the use of these polymers to direct antibodies against fluorescein to self-assembled monolayers (SAMs) presenting d-alanine-d-alanine (dAdA) groups. These polymers bind biospecifically to these
D Samuel et al.
Journal of immunological methods, 107(2), 217-224 (1988-03-16)
Monoclonal antibodies to FITC were produced and shown to be specific for the fluorochrome. Molecular weight marker proteins labelled with FITC could be detected after SDS-PAGE and transfer onto nitrocellulose using anti-FITC followed by an anti-mouse IgG-alkaline phosphatase conjugate. The
S J Rattle et al.
Clinical chemistry, 30(9), 1457-1461 (1984-09-01)
We describe a novel separation procedure for immunoradiometric assays involving monoclonal antibodies in which both radiolabeled and capture antibodies are used in solution, the capture antibody being labeled with fluorescein isothiocyanate (FITC). Separation is achieved by incubation with anti-FITC antibodies
Elsa Charignon et al.
International journal of molecular sciences, 21(21) (2020-10-28)
We describe here the evaluation of the cytotoxic efficacy of two platinum (II) complexes bearing an N-heterocyclic carbene (NHC) ligand, a pyridine ligand and bromide or iodide ligands on a panel of human metastatic cutaneous melanoma cell lines representing different
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