R1158
Ribonuclease Inhibitor
recombinant, expressed in E. coli, 20-40 units/μL
Synonyme(s) :
RNAse inhibitor
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About This Item
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Qualité
Molecular Biology
for molecular biology
Forme
buffered aqueous solution
Poids mol.
~50 kDa
Concentration
20-40 units/μL
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).
Application
Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:
- cDNA synthesis from mRNA
- reverse transcriptase polymerase chain reaction (RT-PCR)
- in vitro transcription and translation
- mRNA protection from degradation
- formaldehyde cross-linked lncRNA purification
- RNA-fluorescence in situ hybridization (FISH)
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
Actions biochimiques/physiologiques
Inhibits RNase by forming a tight, non-covalent 1:1 complex.
Composants
Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.
Définition de l'unité
One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.
Forme physique
Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.
Autres remarques
- Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
- This product is for R&D use only, not for drug, household, or other uses.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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