R7876
Ribonucleic acid, transfer from wheat germ
Type V, 15-19 units/mg solid
Synonyme(s) :
Transfer RNA, tRNA
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About This Item
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Type
Type V
Forme
lyophilized powder
Activité spécifique
15-19 units/mg solid
Conditions de stockage
desiccated
Solubilité
H2O: ~2 mg/mL, clear to hazy
Température de stockage
2-8°C
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Description générale
Wheat germ tRNA contains many different specific tRNA molecules.
Application
Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.
Composants
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Définition de l'unité
One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).
Remplacé(e)(s) par
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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