ORFGFP
MISSION® TRC3 ORF GFP DNA Control
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.51
Conditionnement
vial of 20 μL
Niveau de qualité
Concentration
500 ng/μL
Application(s)
genome mapping
genomic analysis
Conditions d'expédition
dry ice
Température de stockage
−20°C
Catégories apparentées
Description générale
The MISSION TRC3 ORF GFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing TurboGFP™, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome LentiORF Pool. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The GFP Control contains a gene encoding TurboGFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.
In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF GFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The GFP Control contains a gene encoding TurboGFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.
In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF GFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
Functional Genomics/Overexpression Screening/Target Validation
Informations légales
Use of this product is subject to one or more license agreements. For details, please see the MISSION License Page
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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