MAK058
D-Lactate Colorimetric Assay
sufficient for 100 colorimetric tests
Synonyme(s) :
D-Lactate Test Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 100 colorimetric tests
Application(s)
cosmetics
food and beverages
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
neurological disorders; gastrointestinal diseases
Température de stockage
−20°C
Description générale
D-Lactate, typically present in the bloodstream at nanomolar concentrations, is produced by an intestinal source or via the methylglyoxal pathway. In mammals, D-Lactate metabolism requires D-Lactate hydrogenase and is metabolized slowly, thus an increase in blood concentration levels can lead to acidemia and acidosis. The severity of this D-lactic acidosis can be associated with neurotoxic symptoms. Significant D-Lactate accumulations in the body can also be related to impaired metabolism and excretion.
Application
D-Lactate Colorimetric Assay kit has been used to determine the stereospecificity of lactate produced.
Adéquation
Suitable for use with samples of serum, plasma, cells, culture and fermentation media.
Principe
In this assay, D-Lactate is specifically oxidized by D-Lactate hydrogenase and generates a proportional colorimetric product measured at 450 nm. The useful concentration range in samples is 0.1-10 mM D-Lactate.
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Eye Dam. 1 - Skin Corr. 1B
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
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Lucas P Castro et al.
Journal of dairy science, 102(11), 9857-9869 (2019-09-16)
Rehydrated and ensiled mature ground corn has high ruminal starch digestibility, but particle size (PS) and dietary starch proportion (ST) can affect starch digestion and lactating cow performance. We evaluated the effect of rehydrated and ensiled corn (REC), PS, and
Poonam Naik et al.
Current eye research, 45(2), 184-189 (2019-08-31)
Purpose: Microbiological investigations of vitreous fluid have often failed to detect the causative agent in infectious endophthalmitis resulting in a clinical dilemma. D-Lactate is a byproduct of bacterial metabolism, and its accumulation in sterile body fluids indicates bacterial infection. The
Ottavia Benedicenti et al.
Journal of fish diseases, 42(9), 1241-1258 (2019-06-18)
A relationship between increasing water temperature and amoebic gill disease (AGD) prevalence in Atlantic salmon (Salmo salar) has been noted at fish farms in numerous countries. In Scotland (UK), temperatures above 12°C are considered to be an important risk factor
Ayca Erkin-Cakmak et al.
The Journal of clinical endocrinology and metabolism, 104(7), 3003-3011 (2019-03-15)
To investigate the link between dietary sugar consumption and two separate pathogenetic mechanisms associated with metabolic syndrome: de novo lipogenesis (DNL) and nonenzymatic glycation. We assessed changes in serum d-lactate (the detoxification end-product of methylglyoxal) concentration in response to 9
William H. Hoffman et al.
Journal of clinical research in pediatric endocrinology, 12(2), 160-167 (2019-09-14)
To determine the time relationships of soluble receptor for glycation end-products (sRAGE), [a decoy of the advanced glycation end-products (AGE)-RAGE axis] and D-lactate, (a metabolite of methylglyoxal) in the inflammatory response to diabetic ketoacidosis (DKA). Sixteen children and adolescents with
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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