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MAK329

Sigma-Aldrich

L-Lactate Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

L-Lactic Acid Assay Kit, Lactate Quantification Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Entrée

cell
plasma
serum
solution(s) (collagen)

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; cancer

Température de stockage

−20°C

Description générale

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Application

The L-Lactate Assay Kit may be used for:
  • Cancer Research
  • Diabetes Research
  • Endocrinological Disease Research

Caractéristiques et avantages

Sensitive Detection Range: Detect L-Lactate levels accurately across a wide range, from 0.05 mM to 2 mM, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Adéquation

Suitable for lactate determination in serum, plasma, and cell media samples.

Principe

The Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Autres remarques

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Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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