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L9420

Sigma-Aldrich

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Synonyme(s) :

Luciferase firefly

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About This Item

Numéro CAS:
61970-00-1
Numéro de classification (Commission des enzymes):
1.13.12.7 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131546
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Produit recombinant

expressed in E. coli

Niveau de qualité

200

Pureté

≥98% (SDS-PAGE)

Forme

buffered aqueous solution

Activité spécifique

≥10×1010 units/mg protein

Poids mol.

62 kDa

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Application

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Définition de l'unité

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.

Forme physique

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H302,H334,H373

Classification des risques

Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral

Organes cibles

Kidney

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

231.8 °F

Point d'éclair (°C)

111 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Reporter gene-facilitated detection of compounds in Arabidopsis leaf extracts that activate the Karrikin signaling pathway.
Sun Y K, et al.
Frontiers in Plant Science, 7, 1799-1799 (2016)
Greta Forlani et al.
Methods in molecular biology (Clifton, N.J.), 1087, 299-313 (2013-10-26)
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Methods in molecular biology (Clifton, N.J.), 1098, 21-32 (2013-10-30)
Adenosine 5'-triphosphate (ATP) is the "energy currency" of organisms and plays central roles in bioenergetics, whereby its level is used to evaluate cell viability, proliferation, death, and energy transmission. In this chapter, we describe an improved and efficient method for
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