FLE250
Firefly Lantern Extract
for ATP determination
Synonyme(s) :
FLE
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.81
Produits recommandés
Forme
solution
Conditionnement
pkg of 250 mg dried lanterns
Technique(s)
toxicology assay: suitable
Température de stockage
−20°C
Description générale
The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract. The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure. The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.
Application
Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples, bioluminescence assay in river sediments samples, marine sediments and in quantifying extracellular ATP in red blood cells.
Actions biochimiques/physiologiques
Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.
Autres remarques
Crude luciferin and luciferase
Reconstitution
After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Distribution patterns of organic pollutants and microbial processes in marine sediments across a gradient of anthropogenic impact
Zoppini A, et al.
Environmental Pollution (Barking, Essex : 1987), 242, 1860-1870 (2018)
Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions
Marques SM, et al.
IUBMB Life, 61(1), 6-17 (2009)
Oxyluciferin, a luminescence product of firefly luciferase, is enzymatically regenerated into luciferin
Gomi K and Kajiyama N
The Journal of Biological Chemistry, 276(39), 36508-36513 (2001)
Involvement of acetylcholinesterase and protein kinase C in the protective effect of caffeine against beta-amyloid-induced alterations in red blood cells
Carelli-Alinovi C, et al.
Biochimie, 121, 52-59 (2016)
Concentrations and uptake of dissolved organic phosphorus compounds in the Baltic Sea
Nausch M, et al.
Frontiers in Marine Science, 5(386) (2018)
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