L8777
Lectine de Phytolacca americana (phytolaque)
lyophilized powder, BioReagent, suitable for cell culture
Synonyme(s) :
Mitogène de la phytolaque, PWM
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About This Item
Produits recommandés
Stérilité
aseptically filled
Niveau de qualité
Gamme de produits
BioReagent
Forme
lyophilized powder
Puissance
≤0.3 μg/mL mitogenic activity
Technique(s)
cell culture | mammalian: suitable
Solubilité
PBS: 1 mg/mL
Température de stockage
2-8°C
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Catégories apparentées
Description générale
Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.
Application
Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).
Actions biochimiques/physiologiques
Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
Le PWM (mitogène de la phytolaque) est extrait des racines de phytolaque. Cette protéine a des propriétés mitogènes, d′hémagglutination et d′agglutination des leucocytes. Le PWM n′est pas spécifique à un groupe sanguin, mais il présente une affinité pour les oligomères du type N-acétyl-β-D-glucosamine.
Qualité
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Remarque sur l'analyse
L′activité mitogène a été déterminée par incorporation de BrdU dans les cellules HeLa en culture.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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