HPA014600

Anti-MARCH1 antibody produced in rabbit

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• Synonyme(s) : Anti-E3 ubiquitin-protein ligase MARCH1, Anti-MARCH- I, Anti-Membrane-associated RING finger protein 1, Anti-Membrane-associated RING-CH protein I, Anti-RING finger protein 171
• Code UNSPSC : 12352203
• Numéro HPA (Human Protein Atlas): HPA014600
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Gamme de produits: Prestige Antibodies^® Powered by Atlas Antibodies
• Forme: buffered aqueous glycerol solution
• Espèces réactives: human
• Technique(s): immunohistochemistry: 1:50- 1:200
• Séquence immunogène: KVYVQLWRRLKAYNRVIFVQNCPDTAKKLEKNFSCNVNTDIKDAVVVPVPQTGANSLPSAEGG
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MARCH1(55016)

Description

Description générale

MARCH1 (membrane-associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase) is a member of MARCH family of E3 ubiquitin ligases. This family is RING-v type ligases and has 11 members. Most proteins of this family have RING domain in their cytosolic N-terminal, and two transmembrane regions. MARCH1 is generally expressed on secondary lymphoid tissues, such as B-cells and dendritic cells. It is localized to plasma membrane and endosomes.

Immunogène

E3 ubiquitin-protein ligase MARCH1 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Actions biochimiques/physiologiques

MARCH1 (membrane-associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase) is involved in the maturation of dendritic cells. Its under-expression leads to stable surface expression of major histocompatibility (MHC) II, on dendritic cells. Its expression also determines the fate of CD98, post its endocytosis; whether CD98 is degraded or recycled. It is also capable of dimerization and auto-ubiquitination, thus, controlling its own expression. In antigen presenting cells (APCs), its expression is induced by interleukin (IL)-10, which leads to the suppression of MHCII expression. IL-10 is a strong anti-inflammatory cytokine and thus, MARCH1 plays a role in the suppression of immune response. It regulates the cell surface expression of its substrates such as, tumor necrosis factor-related apoptosis inducing ligand (TRAIL) receptor 1. It ubiquitinates and degrades TRAIL-R1, and maintains its steady state expression.

Caractéristiques et avantages

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Liaison

Corresponding Antigen APREST72906

Forme physique

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Informations légales

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)