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H1404

Sigma-Aldrich

Hepatocyte Growth Factor human

>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture

Synonyme(s) :

HGF, Hepatopoietin A, Scatter Factor

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About This Item

Numéro CE :
214-275-1
Numéro MDL:
MFCD00217422
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Nom du produit

Hepatocyte Growth Factor human, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

Source biologique

human

Niveau de qualité

200

Produit recombinant

expressed in Baculovirus infected High-5 cells

Essai

>95% (SDS-PAGE)

Forme

lyophilized powder

Puissance

1.0-20.0 ng/mL

Qualité

endotoxin tested

Poids mol.

~80 kDa

Conditionnement

pkg of 5x5 μg
pkg of 5 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

<1.0 EU/μg

Numéro d'accès UniProt

P14210

Température de stockage

−20°C

Informations sur le gène

human ... HGF(3082)

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Catégories apparentées

Actions biochimiques/physiologiques

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Autres remarques

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH2PO4 and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

Notes préparatoires

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

Remarque sur l'analyse

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
SLCG7933
SLCF4636
SLCD4440
SLCC0104
SLBZ9529

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Masaki Kobayashi et al.
Biochemical and biophysical research communications, 527(1), 153-161 (2020-05-25)
MicroRNA (miRNA) plays a pivotal role in cancer biology. Therefore, tumor suppressor (TS) miRNAs are an attractive target for cancer therapy. However, clinical trials have failed due to the difficulties in miRNA delivery, warranting the development of a novel drug
Ryuichi Tatsumi et al.
American journal of physiology. Cell physiology, 297(2), C238-C252 (2009-06-12)
Regenerative coordination and remodeling of the intramuscular motoneuron network and neuromuscular connections are critical for restoring skeletal muscle function and physiological properties. The regulatory mechanisms of such coordination remain unclear, although both attractive and repulsive axon guidance molecules may be
R A Furlong
BioEssays : news and reviews in molecular, cellular and developmental biology, 14(9), 613-617 (1992-09-01)
Hepatocyte growth factor, a potent mitogen for epithelial and other cell types, and scatter factor, a stimulant of epithelial cell motility are identical. In addition to these mitogenic and motogenic functions, the factor has been shown to be an epithelial
Takanori Takebe et al.
Nature protocols, 9(2), 396-409 (2014-01-25)
Generation of functional and vascularized organs from human induced pluripotent stem cells (iPSCs) will facilitate our understanding of human developmental biology and disease modeling, hopefully offering a drug-screening platform and providing novel therapies against end-stage organ failure. Here we describe
Yingjie Liu et al.
Development (Cambridge, England), 136(5), 843-853 (2009-01-30)
Integrin receptors for the extracellular matrix and receptor tyrosine kinase growth factor receptors represent two of the major families of receptors that transduce into cells information about the surrounding environment. Wnt proteins are a major family of signaling molecules that
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Articles

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

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