GE28-4017-45

GSTrap^™ 4B

Cytiva 28-4017-45, pack of 5 × 1 mL

• Code UNSPSC : 23151816
• Nomenclature NACRES : NA.56

Propriétés

• ligand: glutathione
• Forme: prepacked column
• Réticulation: cross-linked
• Conditionnement: pack of 5 × 1 mL
• Fabricant/nom de marque: Cytiva 28-4017-45
• Conditions de stockage: (20% Ethanol)
• Paramètres: <4 mL/min flow rate; 42 psi (H₂O at 25°C)
• Technique(s): liquid chromatography (LC): suitable
• Taille du lit: 7 mm × 25 mm
• Volume du lit: 1 mL
• D.I. de la colonne: 7 mm
• Matrice: 4% agarose
• Groupe de la matrice active: glutathione functional group
• Taille des particules: 45-165 μm
• Diamètre moyen: 90 μm
• cleaning: 4-13
• working range: 4-13
• Capacité: >25 mg binding capacity (horse liver GST/ml medium)
• Technique de séparation: affinity

Description

Description générale

GSTrap^™ 4B columns are prepacked Glutathione Sepharose^™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

Caractéristiques et avantages

• High binding capacity.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
• GSTrap^™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA^® design.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids