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GE17-0756-05

Glutathione Sepharose 4B

Cytiva 17-0756-05, pack of 100 mL

Synonyme(s) :

affinity chromatography resin, GST-tagged proteins purification

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

glutathione

Conditionnement

pack of 100 mL

Fabricant/nom de marque

Cytiva 17-0756-05

Matrice

4% agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

4-13

working range

4-13

Capacité

>25 mg binding capacity (horse liver GST/ml medium)

Description générale

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Caractéristiques et avantages

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

This page addresses common problems associated with the expression and growth of GST-tagged proteins using GE Healthcare products.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

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Protocoles

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

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