GE17-5474-02

MabSelect SuRe^™ LX

Cytiva 17-5474-02, pack of 200 mL

• Synonyme(s) : Protein purification resin
• Code UNSPSC : 41106500
• Nomenclature NACRES : NA.56

Propriétés

• ligand: alkali-stabilized protein A-derived (E. coli)
• Conditionnement: pack of 200 mL
• Fabricant/nom de marque: Cytiva 17-5474-02
• Conditions de stockage: (20% Ehtanol)
• Matrice: highly cross-linked agarose
• Taille moy. des particules: 85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning in place: 2-13.7
• working range: 3-12
• Capacité: ~60 mg binding capacity
• Adéquation: suitable for bioprocess medium
• Température de stockage: 2-8°C

Description

Description générale

MabSelect^™ SuRe^™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes. Alkali tolerance, high capacity and low ligand leakage in combination with the rigid base matric makes MabSelect^™ SuRe^™ LX highly suitable for purification of monoclonal anitbodies for clinical applications.

MabSelect^™ SuRe^™ LX is a member of the MabSelect^™ family of affinity chromatography media for the capture of monoclonal antibodies (MAbs) at process scale. MabSelect^™ SuRe^™ LX shares features and benefits of MabSelect^™ and MabSelect^™ SuRe^™ such as a rigid, high-flow agarose matrix that ensures excellent pressure/flow properties, low non-specific binding that leads to low impurity levels in the eluate pool, and an oriented ligand coupling for optimal binding capacity. It also features the same alkali-tolerant rProtein A ligand as MabSelect^™ SuRe. This ligand provides greater stability than conventional protein A-based media in the alkaline conditions used in cleaning-in-place (CIP) and sanitization protocols. The enhanced alkali stability of MabSelect^™ SuRe^™ improves process economy and product quality; cleaning can be performed with cost-effective reagents such as sodium hydroxide eliminating the need for expensive and hazardous cleaning agents such as Gua-HCl. Process economy is also improved by the increased dynamic binding capacity of MabSelect^™ SuRe^™ LX.

Just as for MabSelect^™ SuRe, the novel ligand construct of MabSelect^™ SuRe^™ LX has been proven to have an increased stability towards proteases. Therefore, the level of leakage of the MabSelect^™ SuRe^™ LX ligand during elution is low.

MabSelect^™ SuRe^™ has been demonstrated to give generic elution conditions for different monoclonal antibodies which can be applied also to MabSelect^™ SURe LX since it utilizes the same ligand. This is an advantage when designing generic purification platform processes. The feature is also correlated to the construction of the MabSelect^™ SuRe^™ ligand.

MabSelect^™ SuRe^™ LX is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, MabSelect^™ SuRe^™ meets industrial demands with security of supply and comprehensive technical and regulatory support.

human IgG/ml medium. Determined at 10 % breakthrough by frontal analysis at a mobile phase velocity of 100 cm/h in a column with a bed height of 20 cm, i.e. residence time is 6.0 min.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caractéristiques et avantages

• Nouveau ligand (protéine A recombinante) tolérant les bases, résistant aux conditions de stringence des NEP et des désinfections à la soude (NaOH 0,1 M à 0,5 M)
• Réduit le risque de contamination exogène et inter-échantillon
• Améliore les performances tout en diminuant les coûts globaux
• Sa grande capacité de liaison dynamique dans les cultures à titre élevé réduit le temps de traitement et les quantités de milieu utilisées
• Son profil d'élution générique pour différents anticorps monoclonaux permet une approche "plateforme" de la purification
• Sa matrice d'agarose à haut débit permet le traitement de volumes importants

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

Pour consulter le certificat d'analyse de ce produit, rendez-vous sur le site www.cytiva.com.

Informations légales

Ligand du milieu MabSelect SuRe libre
La "licence restreinte sur le ligand de MabSelect SuRe" et la "licence restreinte sur le ligand Cys-rProtéine A" sont protégées par les brevets suivants ainsi que par des brevets et demandes de brevet équivalents dans d'autres pays : US 6 399 750 et EP 1123389. Seuls les produits achetés auprès de Cytiva et de ses distributeurs agréés sont fournis avec une licence limitée, incessible et libre pour l'utilisation de ce produit à des seules fins d'analyses internes. Tout autre usage nécessite l'obtention d'une licence séparée auprès de Cytiva.

MabSelect is a trademark of Cytiva

MabSelect SuRe is a trademark of Cytiva

SuRe is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids