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Key Documents

GE17-0969-01

IgG Sepharose 6 Fast Flow

Cytiva 17-0969-01, pack of 10 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

human IgG

Durée de conservation

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Conditionnement

pack of 10 mL

Fabricant/nom de marque

Cytiva 17-0969-01

Conditions de stockage

(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)

Matrice

highly cross-linked 6% agarose

Diamètre moyen

90 μm

cleaning in place

3-12

working range

3-10

Capacité

~3 mg binding capacity(Protein A)

Température de stockage

2-8°C

Catégories apparentées

Description générale

lgG Sepharose 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it. Medium allows high flow rates to be used for rapid and convenient single step purification of protein.

lgG Sepharose 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.

The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.

Caractéristiques et avantages

  • IgG coupled to the highly cross-linked 6% agarose matrix Sepharose 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
  • Binds at least 2 mg protein A/mL medium
  • Suitable for tandem affinity purification (TAP) of protein complexes

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

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Articles

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocoles

This page shows how to purify protein A-tagged proteins using GE Healthcare products.

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

This page shows how to prepare samples for purification with affinity chromatography.

Contenu apparenté

Tests, réactifs et protocoles permettant d'étudier les interactions protéines/protéines in vitro par différentes méthodes : pull-down ou GST pull-down, purification par affinité en tandem (TAP pour "Tandem Affinity Purification") et co-immunoprécipitation.

Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.

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