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G5922

Sigma-Aldrich

Anti-GW182 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-TNRC6A, Anti-Trinucleotide repeat containing 6A

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

indirect immunofluorescence: 2.5-5.0 μg/mL using human epithelial HEp-2 cells

Numéro d'accès UniProt

Q8NDV7

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Tnrc6a(233833)
rat ... Tnrc6a(308971)

Description générale

GW182 is a phosphorylated cytoplasmic autoantigen. It contains multiple glycine-tryptophan (GW) repeats along with a single RNA recognition motif. GW182 is a protein of 182 kDa, found in mammalian cell types.

Application

Anti-GW182 antibody produced in rabbit has been used as a primary antibody for immunostaining of HeLa cells and human hepatoma cells. It has also been used for indirect immunofluorescence at a working concentration of 2.5-5.0μg/mL using human epithelial HEp-2 cells.

Actions biochimiques/physiologiques

Glycine-tryptophan (GW) bodies or P-bodies are cytoplasmic bodies that are involved in mRNA degradation, storage and translational repression. These bodies contain Argonaute proteins and m-RNAs that associate with GW182. GW182 is involved in post-transcriptional gene silencing/RNA interference (RNAi) via the microRNA pathway. Inhibition of gene expression leads to destruction of GWBs and disruption of RNAi and microRNA-induced gene silencing as it forms an integral component of GWBs.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation
Yang Z, et al.
Journal of Cell Science, 117(23), 5567-5578 (2004)
A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles
Eystathioy T, et al.
Molecular Biology of the Cell, 13(4), 1338-1351 (2002)
Andrew Jakymiw et al.
Journal of cell science, 120(Pt 8), 1317-1323 (2007-04-03)
GW bodies, also known as mammalian P-bodies, are cytoplasmic foci involved in the post-transcriptional regulation of eukaryotic gene expression. Recently, GW bodies have been linked to RNA interference and demonstrated to be important for short-interfering-RNA- and microRNA-mediated mRNA decay and
Andrew Jakymiw et al.
Nature cell biology, 7(12), 1267-1274 (2005-11-15)
The GW182 RNA-binding protein was initially shown to associate with a specific subset of mRNAs and to reside within discrete cytoplasmic foci named GW bodies (GWBs). GWBs are enriched in proteins that are involved in mRNA degradation. Recent reports have
Zheng Yang et al.
Journal of cell science, 117(Pt 23), 5567-5578 (2004-10-21)
A novel cytoplasmic compartment referred to as GW bodies was initially identified using human autoantibodies to a 182 kDa protein named GW182. GW bodies are small, generally spherical, cytoplasmic domains that vary in number and size in several mammalian cell
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