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G5507

Sigma-Aldrich

Galactosyltransferase from bovine milk

lyophilized powder

Synonyme(s) :

Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase

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About This Item

Numéro CAS:
9031-68-9
Numéro de classification (Commission des enzymes):
2.4.1.22 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131122
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

200

Activité spécifique

3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)

Composition

Protein, 35-65% modified Warburg-Christian

Impuretés

≤10 % protein α-lactalbumin

Température de stockage

−20°C

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Description générale

Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.

Application

Galactosyltransferase from bovine milk has been used:
  • in the generation of hyper-galactosylated variant of IgG1
  • in β -1,4-galactosyltransferase assay rice root cell extracts
  • in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

Actions biochimiques/physiologiques

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides.
Galactosyltransferase from bovine milk requires Mn2+ ions for its catalytic functionality. Alteration in the galactosyltransferase levels have been implicated in various diseases states.

Liaison

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

Définition de l'unité

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

Forme physique

Lyophilized powder containing Tris, EDTA, and (NH4)2SO4

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Structure and function of beta-1, 4-galactosyltransferase
Qasba PK, et al.
Current Drug Targets, 9(4), 292-309 (2008)
Hierarchical self-assembly and emergent function of densely glycosylated peptide nanofibers
Restuccia A, et al.
Communications Chemistry, 2(1), 53-53 (2019)
In vitro glycoengineering of IgG1 and its effect on Fc receptor binding and ADCC activity
Thomann M, et al.
PLoS ONE, 10(8), e0134949-e0134949 (2015)
A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities.
J A Campbell et al.
The Biochemical journal, 326 ( Pt 3), 929-939 (1997-10-23)
A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice
Jiang H, et al.
Plant Physiology, 138(1), 232-242 (2005)
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Articles

Glycosyltransferases

Glycosyltransferases were initially considered to be specific for a single glycosyl donor and acceptor, which led to the one enzyme-one linkage concept. Subsequent observations have refuted the theory of absolute enzymatic specificity by describing the transfer of analogs of some nucleoside mono- or diphosphate sugar donors.

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