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G4548

Sigma-Aldrich

GlycoProfile™ Glycan Clean-Up Cartridge

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Catégories apparentées

Description générale

Glycan analysis has become an increasingly critical aspect of glycomics and proteomics, due to the role of glycoproteins in cell signaling, cell adhesion, immune response, and disease states. Since they tend to have low spectral activity in both UV and visible light, it is often necessary to label glycans in order to enhance detection. GlycoProfile™ Glycan Clean-Up Cartridges are designed for the post-labeling cleanup of labelled glycans when using the GlycoProfile™ 2-AA or 2-AB Labeling Kits. Glycans adsorb to the membrane while excess dye and other hydrophobic contaminants pass through. Mono- and disaccharides have a weak affinity for the cartridge membrane and tend to bind poorly. Purified glycans are subsequently desorbed and ready for further enzymatic digestion or analysis by a number of methods, including HPLC.

Application

GlycoProfile™ Glycan Clean-Up Cartridge is used for clean up of glycan samples after reductive amination labeling or enzymatic digestion. It has also been used to remove excess dye and hydrophobic contaminants from N-linked glycans. The cartridge is equilibrated with acetonitrile, then loaded with a maximum of 20 μg of a glycan sample. The glycans are bound while excess dye and salts are removed by washing with acetonitrile. The glycans are then eluted with water.

Liaison

Recommended for use with the GlycoProfile 2-AA (Cat. No. PP0530) and 2-AB (Cat. No. PP0520) Labeling Kits.

Stockage et stabilité

Store the cartridges at room temperature. The cartridges are stable for at least one year at room temperature, as supplied.

Autres remarques

Each cartridge is recommended for single use only. The maximum sample size is 10 μL of glycan containing solution and/or 20 μg of glycan.

Informations légales

GlycoProfile is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
GlycoMass is a trademark of Sigma-Aldrich Co. LLC
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Clause de non-responsabilité

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Preclinical Characterization of CTL-1,
A Biosimilar Anti-EGFR Monoclonal
Antibody for Cetuximab
Hu P, et al.
Journal of Chemical and Pharmaceutical Sciences, 1(1), 1003-1003 (2017)
Substitution of glutamine by glutamate enhances production and galactosylation of recombinant IgG in Chinese hamster ovary cells
Hong JK, et al.
Applied Microbiology and Biotechnology, 88(4), 869-876 (2010)
Recent trends in analytical and structural glycobiology
Novotny MV, et al.
Current Opinion in Chemical Biology, 17(5), 832-840 (2013)

Articles

Glycoprofile™ Labeling Kits

Glycoprofile Labeling Kits for Glycan Analysis designed for efficient labeling of N-linked, O-linked and glycosylphosphatidylinositol (GPI) anchored glycans using your choice of 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA) 1,2

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