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Key Documents

F2016

Sigma-Aldrich

Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Conjugué

FITC conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Conditions de stockage

protect from light

Technique(s)

indirect immunofluorescence: 1:160 using mouse splenocytes

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Binds all goat Igs.
IgG (immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.

Application

Anti-Goat IgG (whole molecule)-FITC antibody produced in rabbit has been used in immunofluorescence to study the translocation of CXCR4 in human hepatoma cell lines. It has also been used in immunocytochemistry.

Actions biochimiques/physiologiques

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Goat IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Autres remarques

Antibody adsorbed with human serum proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Notes préparatoires

Adsorbed to reduce background staining with human samples.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Lin Song et al.
Stem cells and development, 14(6), 712-721 (2006-01-26)
Much of the knowledge regarding the regulatory pathways for adult stem cell self-renewal and differentiation has been obtained from the results of in vitro cultures. However, it is unclear if adult stem cells are controlled in the same way under
Jeremiah Morrissey et al.
Journal of the American Society of Nephrology : JASN, 11(9), 1681-1690 (2000-08-31)
Analysis of gene expression in a mouse model of unilateral ureteral obstruction (UUO) revealed significant induction of CD14 mRNA in kidneys with obstructed ureters. Immunocytochemical analysis indicated that CD14 was upregulated in tubular epithelial cells and this upregulation was not
C C Schimanski et al.
British journal of cancer, 95(2), 210-217 (2006-07-05)
In different tumour entities, expression of the chemokine receptor 4 (CXCR4) has been linked to tumour dissemination and poor prognosis. Therefore, we evaluated, if the expression of CXCR4 exerts similar effects in human hepatocellular carcinoma (HCC). Expression analysis and functional
C D Gregory et al.
The Journal of general virology, 71 ( Pt 7), 1481-1495 (1990-07-01)
Epstein-Barr virus (EBV)-positive Burkitt's lymphoma (BL) biopsy cells and early passage BL cell lines have been reported as showing an unusual type of virus-cell interaction; at least two EBV latent proteins appear not to be expressed. Serial passage of such
Marta de Almeida Santiago et al.
PloS one, 11(12), e0167669-e0167669 (2016-12-10)
The use of antibodies in immunodiagnostic kits generally implies the conjugation of these proteins with other molecules such as chromophores or fluorochromes. The development of more sensitive quality control procedures than spectrophotometry is essential to assure the use of better

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