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E1388

Sigma-Aldrich

β-Endothelial Cell Growth Factor human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

ECGS, β-ECGF

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About This Item

Numéro CAS:
105843-51-4
Numéro MDL:
MFCD00131012
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

β-Endothelial Cell Growth Factor human, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥97% (SDS-PAGE)

Forme

lyophilized powder

Puissance

0.03-0.3 ng/mL ED50/EC50

Qualité

endotoxin tested

Poids mol.

predicted mol wt ~17 kDa

Conditionnement

pkg of 25 μg

Conditions de stockage

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1.0 EU/μg protein Endotoxin level (LAL test)

Numéro d'accès UniProt

P49765

Température de stockage

−20°C

Informations sur le gène

human ... VEGFB(7423)

Catégories apparentées

Description générale

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

Application

β-Endothelial Cell Growth Factor human has been used:
  • in cell death assay
  • in endothelial cell culture
  • in human umbilical vascular endothelial cell (HUVEC) culture

Actions biochimiques/physiologiques

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

Autres remarques

14 amino acid, N-terminal extended form of human FGF-acidic.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Remarque sur l'analyse

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

A blood pressure-associated variant of the SLC39A8 gene influences cellular cadmium accumulation and toxicity
Zhang R, et al.
Human Molecular Genetics, 25(18), 4117-4126 (2016)
Serum vascular endothelial growth factor B is elevated in women with polycystic ovary syndrome and can be decreased with metformin treatment
Cheng F, et al.
Clinical Endocrinology, 84(3), 386-393 (2016)
Eleanor G Lynam et al.
Methods in molecular biology (Clifton, N.J.), 2475, 223-228 (2022-04-23)
Endothelial cell proliferation rate is an important indicator of vascular health. Being able to detect the rate of endothelial cell proliferation, or cell cycle disturbances after intervention is a valuable tool for analysing any beneficial or detrimental effects of treatments
Chuan-Rong Zhao et al.
Small (Weinheim an der Bergstrasse, Germany), 19(2), e2204694-e2204694 (2022-11-21)
Disturbed blood flow induces endothelial pro-inflammatory responses that promote atherogenesis. Nanoparticle-based therapeutics aimed at treating endothelial inflammation in vasculature where disturbed flow occurs may provide a promising avenue to prevent atherosclerosis. By using a vertical-step flow apparatus and a microfluidic
Bioinformatics analyses of the role of vascular endothelial growth factor in patients with non-small cell lung cancer
Wang Y, et al.
PLoS ONE, 10(9), e0139285-e0139285 (2015)
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