E0760

Endothelial cell growth supplement from bovine pituitary

ECGS, suitable for cell culture, BioReagent

• Synonyme(s) : ECGS, Endothelial Mitogen
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: bovine pituitary glands
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: powder
• Puissance: 2-300 μg/mL ED₅₀/EC₅₀
• Qualité: endotoxin tested
• Conditionnement: pkg of 15 mg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤20 EU/vial (LAL test)
• Couleur: brown to tan
• Solubilité: water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)
• Température de stockage: 2-8°C

Description

Description générale

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:

• as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
• as a supplement in medium 199 for endothelial cell culture
• as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
• as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Actions biochimiques/physiologiques

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H361fd
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Repr. 2
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable