CS1020
Glutathione Assay Kit, Fluorimetric
sufficient for 200 multiwell tests
Synonyme(s) :
GSH Quantification Kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Utilisation
sufficient for 200 multiwell tests
Niveau de qualité
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... GSTA4(2941)
Application
Glutathione Assay Kit, Fluorimetric has been used in intracellular thiol assays and in the measurement of hepatic glutathione in rats.
The Glutathione Assay Kit, Fluorimetric includes all the reagents required for fast and easy measurement of reduced glutathione in cell or tissue extracts or directly in growing cells. Staurosporine is supplied with the kit to enable induction of apoptosis in cells. Since the amount of reduced glutathione in staurosporine-induced cells is significantly lower than in non-induced cells, this can serve as a control system.
Actions biochimiques/physiologiques
Glutathione (GSH) acts as a cofactor for several antioxidant enzymes. It helps in the regeneration of vitamins C and E. It regulates cellular proliferation and apoptosis. GSH is important for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene is associated with Alzheimer′s, Parkinson′s and Huntington′s diseases.
Reduced glutathione (GSH), a tripeptide (γ-glutamyl-cysteinylglycine), is the major free thiol in most living cells and is involved in many biological processes such as detoxification of xenobiotics, removal of hydroperoxides, and maintenance of the oxidation state of protein sulfhydryls. It is the key antioxidant in animal tissues. Glutathione is present inside cells primarily in the reduced form (90-95% of the total glutathione). The remainder is present in the oxidized form (glutathione disulfide, GSSG). Intracellular GSH status appears to be a sensitive indicator of the overall health of a cell and its ability to resist toxic challenge. High levels of GSH in the cell may indicate pathological changes.
Remarque sur l'analyse
The kit assay utilizes a thiol probe (monochlorobimane), which can freely pass through the plasma membrane. The free, unbound probe shows very little fluorescence, but when bound to reduced glutathione in a reaction that is catalyzed by glutathione S-transferase (GST), it forms a strongly fluorescent adduct.
Composants de kit seuls
Réf. du produit
Description
- Assay Buffer 50 mL
- Substrate Solution 1 mL
- Lysis Buffer 10x 1.5 mL
- Glutathione S-Transferase 1 mL
- Staurosporine Ready Made 100 μL
- Glutathione, reduced 300 mg
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Mehdi Hichor et al.
Scientific reports, 8(1), 2524-2524 (2018-02-08)
Reactive oxygen species (ROS) modify proteins and lipids leading to deleterious outcomes. Thus, maintaining their homeostatic levels is vital. This study highlights the endogenous role of LXRs (LXRα and β) in the regulation of oxidative stress in peripheral nerves. We
Protective effects of Korean red ginseng extract on cadmium-induced hepatic toxicity in rats
Park SJ, et al.
Journal of ginseng research, 37(1), 37-37 (2013)
Susan García Fillería et al.
Food chemistry. Molecular sciences, 3, 100053-100053 (2022-04-14)
The relationship between structural and physicochemical properties and antioxidant activity of peptides from amaranth 11S-globulin was studied. Peptides AWEEREQGSR, TEVWDSNEQ, IYIEQGNGITGM and YLAGKPQQEH had the greatest in vitro activity (ORAC, HORAC). GDRFQDQHQ, HVIKPPSRA and KFNRPETT were the most active ones
Boutaina Daher et al.
Cancers, 14(13) (2022-07-10)
The conceptualization of a novel type of cell death, called ferroptosis, opens new avenues for the development of more efficient anti-cancer therapeutics. In this context, a full understanding of the ferroptotic pathways, the players involved, their precise role, and dispensability
Glutathione: Physiological and clinical relevance
Pizzorno JE and Katzinger JJ
Journal of restorative medicine, 1(1), 24-37 (2012)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique