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Safe Harbor Landing Pad Cell Line A375 Cancer Cells

human female skin (Source Disease: Malignant melanoma)

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

Safe Harbor Landing Pad Cell Line A375 Cancer Cells,

Source biologique

human female skin (Source Disease: Malignant melanoma)

Niveau de qualité

Mode de croissance

Adherent

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

dry ice

Température de stockage

−196°C

Comparer avec des articles similaires

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1 of 4

Cet article
HPA030817WH0055823M1SAB1405175
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Gene Information

human ... YKT6(10652)

Gene Information

human ... YKT6(10652)

Gene Information

human ... VPS11(55823)

Gene Information

human ... BIN3(55909)

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

human

species reactivity

human

species reactivity

mouse

species reactivity

human

Description générale

The STR profile of this cell line matches that of its parental cell line ATCC® Catalog No. CRL-1619. A375 are a human malignant melanoma cell line isolated from a 54 year old female. The cells possess a hypotriploid karyotype.

Description de la lignée cellulaire

These cells are a human malignant melanoma cell line isolated from a 54-year old female. The cells possess a hypotriploid karyotype.

Application

This product is a human A375 melanoma cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Caractéristiques et avantages

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These A375 cells are adherent with a doubling time of approximately 22 hours.

Qualité

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Milieu de culture

DMEM (Catalog Number D6429) with 10% FBS (Catalog Number F2442)

Informations légales

ATCC is a registered trademark of American Type Culture Collection
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Kimi Araki et al.
Nucleic acids research, 30(19), e103-e103 (2002-10-05)
The Cre-lox system is an important tool for genetic manipulation. To promote integrative reactions, two strategies using mutant lox sites have been developed. One is the left element/right element (LE/RE)-mutant strategy and the other is the cassette exchange strategy using
Huseyin Tas et al.
PloS one, 10(9), e0136963-e0136963 (2015-09-04)
We describe an optimized system for the easy, effective, and precise modification of the Escherichia coli genome. Genome changes are introduced first through the integration of a 1.3 kbp Landing Pad consisting of a gene conferring resistance to tetracycline (tetA)
Zhong-Wei Du et al.
Stem cells (Dayton, Ohio), 27(5), 1032-1041 (2009-05-06)
To circumvent the silencing effect of transgene expression in human embryonic stem cells (hESCs), we employed the Cre recombination-mediated cassette exchange strategy to target the silencing-resistant site in the genome. We have identified new loci that sustain transgene expression during

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