M15

95102517, mouse kidney, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: M15, 95102517
• Source biologique: mouse kidney
• Mode de croissance: Adherent
• Caryotype: Hyperdiploid
• Morphologie: Epithelial
• Produits: WT1 (expressed gene)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse mesonephric epithelium, polyoma virus large T transformed

Description de la lignée cellulaire

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

Application

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

Autres remarques

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