07-405

Anti-PRMT5 Antibody

Upstate^®, from rabbit

• Synonyme(s) : 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog, SKB1 homolog (S. pombe), Shk1 kinase-binding protein 1 homolog, protein arginine methyltransferase 5, skb1 (S. pombe) homolog
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse, human
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NM_006109
• Numéro d'accès UniProt: O14744
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PRMT5(10419)

Description

Description générale

Arginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.

PRMT5 (aka JBP1 Janus Binding Protein1) is a member of a family of enzymes that deposit methyl groups on arginine residues of proteins, including both histone and non-histone substrates.

Spécificité

PRMT5, Mr ~70 kDa

Immunogène

KLH-conjugated, synthetic peptide corresponding to amino acids 60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.

Application

Anti-PRMT5 Antibody is a Rabbit Polyclonal Antibody for detection of PRMT5 also known as 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog & has been validated in IP & WB.

Immunoprecipitation:
An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualité

Routinely evaluated by Western Blot in RIPA lysates from 3T3/A31, NIH 3T3,HeLa, WI-38, and Jurkat cells.

Western Blot Analysis:
0.5-1 μg/mL of this lot detected PRMT5 in RIPA lysates from 3T3/A31, NIH 3T3, HeLa, WI-38, and Jurkat cells.

Description de la cible

~70 kDa

Liaison

Replaces: MABE551

Forme physique

Format: Purified

Protein A purified

Purified rabbit IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Remarque sur l'analyse

Control
Jurkat cell lysate, HeLa cell lysate.

Included Positive Antigen Control:
Catalog # 12-303, Jurkat lysate. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1