B16-F1 Cell Line from mouse

92101203, mouse skin, Fibroblast-like

• Code UNSPSC : 41106514

Propriétés

• product name: B16-F1 Cell Line from mouse, 92101203
• Source biologique: mouse skin
• Description: Mouse melanoma, producing melanin
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast-like
• Produits: Melanin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse melanoma, producing melanin

Description de la lignée cellulaire

Mouse melanoma which produces melanin.

Application

B16-F1 cell line from mouse has been used to study the cytotoxic and antioxidant properties of supercritical CO2 extract (SCE) of old man′s beard (Usnea barbata). It has also been used to study the antiproliferative activity of essential oils from Achillea ligustica.

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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