NIH 3T3 Cell Line murine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93061524, mouse embryo, Fibroblast

• Synonyme(s) : 3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse embryo
• Description: Embryonic fibroblast
• Conditionnement: tube of 5 μg 93061524-DNA-5UG; pkg of vial of cells 93061524-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse Swiss NIH embryo

Description de la lignée cellulaire

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.

Application

DNA transfection studies

NIH 3T3 Cell Line has been used to:

• study calcium-mediated actin reset (CaAR) in response to physiological changes
• study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
• determine the viral titer of the retroviral construct -ST40hTNFα

Milieu de culture

DMEM + 2mM Glutamine + 10% Calf Serum (CS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

Autres remarques

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