TT

92050721, human thyroid, Not specified

• Code UNSPSC : 41106514

Propriétés

• product name: TT, 92050721
• Source biologique: human thyroid
• Mode de croissance: Adherent
• Caryotype: Aneuploid karyotype with marker chromosomes
• Morphologie: Not specified
• Produits: Calcitonin, Carcinoembryonic antigen (CEA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian thyroid medullary carcinoma

Description de la lignée cellulaire

Established from a thyroid medullary carcinoma of a 77 year old Caucasian female. Tumourigenic in nude mice.

Application

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18

Milieu de culture

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Population doubling is very slow, cells can take up to 5 days to reach 50% confluency; media change 2-3 times a week is recommen

Autres remarques

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