WI 38VA13 Subline 2RA

85062512, human lung (fetal), Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: WI 38VA13 Subline 2RA, 85062512
• Source biologique: human lung (fetal)
• Mode de croissance: Adherent
• Caryotype: 2n = 46, hyperdiploid, modal no. 73-78
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian foetal lung, SV40 transformed

Description de la lignée cellulaire

An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.

Application

Transformation and virus studies: SV40

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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