L929/R

4102001, mouse adipose tissue, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: L929/R, 04102001
• Source biologique: mouse adipose tissue
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse C3H/An connective tissue cisplatin resistant

Description de la lignée cellulaire

This cisplatin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (Sigma Catalogue number. 85011425) to increasing concentrations of cisplatin in vitro. L929/R cells can be used in the development of novel anti-cancer treatments. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

Application

Development of novel anti-cancer treatments

Milieu de culture

RPMI 1640 + 2mM L-Glutamine + 10% Foetal Bovine Serum (FBS) + 0.26μM Cisplatin.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:5 to 1:50 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 2% CO₂; 37 C.

Autres remarques

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