3T3-F442A
70654, mouse adipose tissue, Fibroblast-like
Synonyme(s) :
3T3F442A Cells, F442A Cells
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About This Item
Code UNSPSC :
41106514
Produits recommandés
product name
3T3-F442A, 00070654
Source biologique
mouse adipose tissue
Mode de croissance
Adherent
Caryotype
Not specified
Morphologie
Fibroblast-like
Produits
Not specified
Récepteurs
Not specified
Technique(s)
cell culture | mammalian: suitable
Conditions d'expédition
dry ice
Température de stockage
−196°C
Origine de la lignée cellulaire
Mouse pre-adipocytes
Description de la lignée cellulaire
Cells will differentiate into adipocytes once confluent which takes approximately 10 days. Once confluent, cells should be grown in DMEM and 10% FCS and 5 micrograms/ml insulin. Media changes should take place every 48 h.
To manage customer expectations regarding the potential of 3T3 cell line stocks to differentiate into adipocytes, if using the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g., 2 - 5 weeks, and the proportion of the population that differentiates can be limited. If 3T3 cells from an alternate source were previously used, we cannot guarantee the differentiation performance will be the same.We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.
To manage customer expectations regarding the potential of 3T3 cell line stocks to differentiate into adipocytes, if using the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g., 2 - 5 weeks, and the proportion of the population that differentiates can be limited. If 3T3 cells from an alternate source were previously used, we cannot guarantee the differentiation performance will be the same.We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.
Application
Once terminally differentiated the cells can be used as a model for either adipocyte differentiation or mature adipocytes.
Milieu de culture
Procédure de repiquage
Split sub-confluent cultures (70-80%) 1:3-1:5 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA, 5% CO2, 37°C. If cells are allowed to become confluent they will differentiate into adipocytes. If cryopreserving these cells, use New
Autres remarques
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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