C7624
Collagen human
Bornstein and Traub Type I, recombinant, expressed in Nicotiana tabacum (tobacco)
Synonyme(s) :
Collage™
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About This Item
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in Nicotiana tabacum (tobacco)
Pureté
>90% (SDS-PAGE)
Forme
liquid
Concentration
2.5-3.5 mg/mL protein (Sircol method)
Technique(s)
cell culture | mammalian: suitable
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Informations sur le gène
human ... COL1A1(1277) , COL1A2(1278)
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Description générale
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Forme physique
Supplied as a filter-sterilized liquid in 10 mM HCl. Do not freeze.
Notes préparatoires
Coating of tissue culture dishes: Use 3 to 10 microgram per 1 cm2 dish area. For each cell type and plate type an optimization may be required. Dilute in 10 mM HCl or PBS. Apply the diluted collagen to a tissue culture plate and allow to dry overnight in a laminar flow hood with the plate lid open. Aspirate gently the remaining liquid from the coated wells. Wash twice gently with PBS.
To prepare a collagen gel the fibrillogenesis buffer contains 162 mM sodium phosphate dibasic (Na2HPO4) adjusted to pH 11.2 with 10 N NaOH, filter sterilized. Mix 9 volumes of Collagen at a concentration of 0.3%-1% with 1 volume of fibrillogenesis buffer. Mix well and incubate for 4 to 16 hours at 25°C to 27 °C. Higher concentration of collagen solution might need optimization. Thiis preparation will not spontaneously form a gel in PBS or cell culture medium.
Preparation of collagen sponge: concentrate gel to 10 mg/ml - 100 mg/ml by centrifugation, freeze dry. Crosslink by common methods found in the scientific literature such as 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), Glutaraldehyde or thermal dehydration.
Other forms: membranes, sheets, fibers can be prepared by methods commonly used with other types of collagen.
To prepare a collagen gel the fibrillogenesis buffer contains 162 mM sodium phosphate dibasic (Na2HPO4) adjusted to pH 11.2 with 10 N NaOH, filter sterilized. Mix 9 volumes of Collagen at a concentration of 0.3%-1% with 1 volume of fibrillogenesis buffer. Mix well and incubate for 4 to 16 hours at 25°C to 27 °C. Higher concentration of collagen solution might need optimization. Thiis preparation will not spontaneously form a gel in PBS or cell culture medium.
Preparation of collagen sponge: concentrate gel to 10 mg/ml - 100 mg/ml by centrifugation, freeze dry. Crosslink by common methods found in the scientific literature such as 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), Glutaraldehyde or thermal dehydration.
Other forms: membranes, sheets, fibers can be prepared by methods commonly used with other types of collagen.
Informations légales
Collage is a trademark of CollPlant Ltd.
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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