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B5525

Sigma-Aldrich

Bromophenol Blue sodium salt

Synonyme(s) :

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

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About This Item

Formule empirique (notation de Hill):
C19H9Br4NaO5S
Numéro CAS:
34725-61-6
Poids moléculaire :
691.94
Numéro Beilstein :
6995701
Numéro CE :
252-170-2
Numéro MDL:
MFCD00013793
Code UNSPSC :
12171500
ID de substance PubChem :
57653941
Nomenclature NACRES :
NA.52

Forme

powder

Niveau de qualité

200

Technique(s)

electrophoresis: suitable

Solubilité

water: soluble

Adéquation

suitable for nucleic acid electrophoresis

Température de stockage

room temp

Chaîne SMILES 

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

Clé InChI

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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Catégories apparentées

Description générale

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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