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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

Synonyme(s) :

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form

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About This Item

Formule empirique (notation de Hill):
C19H10Br4O5S
Numéro CAS:
115-39-9
Poids moléculaire :
669.96
Numéro Beilstein :
61698
Numéro CE :
204-086-2
Numéro MDL:
MFCD00005875
Code UNSPSC :
12171500
ID de substance PubChem :
24847169
Nomenclature NACRES :
NA.47

Qualité

ACS reagent

Niveau de qualité

200

Forme

powder

Technique(s)

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

Pf

273 °C (lit.)

Solubilité

ethanol: 0.1%

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

Clé InChI

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Catégories apparentées

Description générale

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCG4635
BCCG0495
BCCD6533
SHBL2679
SHBL1423

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ibrahim Alkatout et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 28(1), 69-79 (2014-06-21)
The prognosis of pancreatic neuroendocrine tumors is related to size, histology and proliferation rate. However, this stratification needs to be refined further. We conducted a proteome study on insulinomas, a well-defined pancreatic neuroendocrine tumor entity, in order to identify proteins
Dana Westphal et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(39), E4076-E4085 (2014-09-18)
The pivotal step on the mitochondrial pathway to apoptosis is permeabilization of the mitochondrial outer membrane (MOM) by oligomers of the B-cell lymphoma-2 (Bcl-2) family members Bak or Bax. However, how they disrupt MOM integrity is unknown. A longstanding model
Helen Karlberg et al.
The Journal of general virology, 96(Pt 3), 538-546 (2014-12-08)
Pathogenesis of viral haemorrhagic fevers is associated with alteration of vascular barrier function and haemorrhage. To date, the specific mechanism behind this is unknown. Programmed cell death and regulation of apoptosis in response to viral infection is an important factor
Stephanie L Smith-Roe et al.
Oncotarget, 6(2), 732-745 (2014-12-30)
SWI/SNF complexes utilize BRG1 (also known as SMARCA4) or BRM (also known as SMARCA2) as alternative catalytic subunits with ATPase activity to remodel chromatin. These chromatin-remodeling complexes are required for mammalian development and are mutated in ~20% of all human
Siao-Syun Guan et al.
Oncotarget, 5(13), 4868-4880 (2014-06-21)
Colorectal cancer (CRC) is known as a common malignant neoplasm worldwide. The role of EGFR/HER2 in CRC is unclear. Afatinib is an irreversible EGFR/HER2 inhibitor. There were few studies of afatinib on CRC. Here, we investigated the protein levels/expressions of
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