B3931

Bisindolylmaleimide X hydrochloride

≥90%, solid

• Synonyme(s) : Ro 31-8425
• Formule empirique (notation de Hill): C₂₆H₂₄N₄O₂ · HCl
• Numéro CAS: 145317-11-9
• Poids moléculaire : 460.96
• Numéro MDL: MFCD04972010
• Code UNSPSC : 12352111
• ID de substance PubChem : 329773224
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: synthetic (organic)
• Niveau de qualité: 200
• Pureté: ≥90%
• Forme: solid
• Solubilité: DMSO: soluble
• Température de stockage: −20°C
• Chaîne SMILES: Cl.Cn1cc(C2=C(C(=O)NC2=O)c3c4CC(CN)CCn4c5ccccc35)c6ccccc16
• InChI: 1S/C26H24N4O2.ClH/c1-29-14-18(16-6-2-4-8-19(16)29)23-24(26(32)28-25(23)31)22-17-7-3-5-9-20(17)30-11-10-15(13-27)12-21(22)30;/h2-9,14-15H,10-13,27H2,1H3,(H,28,31,32);1H
• Clé InChI: IMBOYWXMTUUYGZ-UHFFFAOYSA-N

Description

Application

Bisindolylmaleimide X hydrochloride has been used as a protein kinase C (PKC) inhibitor:

• in chemotaxis assays
• to inhibit protein kinase C and to study its effects on the expression of EGR1, NAB2, ZEBRA, and Rta
• in the culture to study its effects on the expression of the kinase-insert domain-containing receptor (KDR)-B1, protein kinase Cθ (PKCθ)-M1a or Abl-HTa and on cell yield in baculovirus (BV)-infected insect cells

Actions biochimiques/physiologiques

Bisindolylmaleimide X hydrochloride/Ro 31-8425, a strong and selective protein kinase C (PKC) inhibitor, can reduce the superoxide burst caused by various agonists in neutrophils. It can suppress the responses induced by cell surface receptors and phorbol esters in T cells. In humans, Ro 31-8425 can prevent neutrophil PKC in vitro with an IC50 of 5nM.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)