A2917

Acrylamide/Bis-acrylamide

for molecular biology, BioReagent, 19:1 (ratio)

• Synonyme(s) : Acrylamide/Bisacrylamide, 19:1 (5% crosslinker), Polyacrylamide solution
• Numéro MDL: MFCD00080848
• Code UNSPSC : 41105319
• ID de substance PubChem : 329770804
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: powder
• Ratio alimentaire: 19:1 (ratio)
• Activité étrangère: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• Chaîne SMILES: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• Clé InChI: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Description

Description générale

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide has been used:

• in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP)
• in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping
• in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Caractéristiques et avantages

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H301,H312 + H332,H315,H317,H319,H340,H350,H361fd,H372
• Conseils de prudence: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Organes cibles: Peripheral nervous system
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges