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Key Documents

909475

Sigma-Aldrich

5-Ethynyl uridine

≥95%

Synonyme(s) :

5-EU, EU

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About This Item

Formule empirique (notation de Hill):
C11H12N2O6
Numéro CAS:
Poids moléculaire :
268.22
Code UNSPSC :
41106305
Nomenclature NACRES :
NA.22

Pureté

≥95%

Forme

powder

Capacité de réaction

reaction type: click chemistry

Température de stockage

−20°C

InChI

1S/C11H12N2O6/c1-2-5-3-13(11(18)12-9(5)17)10-8(16)7(15)6(4-14)19-10/h1,3,6-8,10,14-16H,4H2,(H,12,17,18)/t6-,7-,8-,10-/m1/s1

Clé InChI

QCWBIPKYTBFWHH-FDDDBJFASA-N

Application

This alkyne-bearing metabolic labeling reagent can be used to measure RNA synthesis in proliferating cells. Once fed to cells, this nucleoside is incorporated during active RNA synthesis and can then be detected using a copper-catalyzed click reaction with fluorescent dyes. The cells can then be analysed by fluorescence imaging, flow cytometry or high content imaging analysis.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs
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