76704

modified Tryptic Soy Broth- Dehydrated Culture Media

NutriSelect^® Plus, novobiocin, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC)

• Synonyme(s) : Modified Tryptic Soy Broth with Novobiocin, Modified Tryptic Soy Broth; EHEC enrichment broth, mTSB Broth with Novobiocin
• Code UNSPSC : 41171607
• Nomenclature NACRES : NA.85

Propriétés

• Agence: according to ISO 16654:2001
• Niveau de qualité: 200
• Stérilité: non-sterile
• Forme: powder
• Durée de conservation: limited, expiry date on the label
• Composition: bile salts No. 3, 1.5 g/L ; di-potassium hydrogen phosphate, 4.0 g/L ; D(+)-glucose, 2.5 g/L ; novobiocin ; peptone from casein, 17.0 g/L ; peptone from soymeal, 3.0 g/L ; sodium chloride, 5.0 g/L
• Fabricant/nom de marque: NutriSelect^® Plus
• Conditionnement: pkg of -500 g
• Conditions de stockage: (Dry place, tightly sealed containers)
• Technique(s): microbiological culture: suitable
• Couleur: faint brown
• pH final: 7.3±0.2 (25 °C)
• Application(s): agriculture; food and beverages; microbiology; pathogen testing
• Température de stockage: 2-25°C
• Adéquation: selective for Escherichia coli; selective for coliforms; selective for enterovirulent E. coli (EEC)

Description

Description générale

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Application

mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC).

Notes préparatoires

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

Note de bas de page

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)