CSPD-RO
Roche
CSPD ready-to-use
Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate
Synonyme(s) :
ready-to-use, cspd
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41105500
Produits recommandés
Niveau de qualité
Pureté
>98% (HPLC)
Forme
solution
Poids mol.
Mr 461
Conditionnement
pkg of 1 mL (11655884001[25mM])
pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use)
Fabricant/nom de marque
Roche
Température de stockage
2-8°C
Description générale
CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
Application
CSPD ready-to-use has been used for:
- the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)
- colony and plaque hybridization
- gel shift assay
- sequencing
Caractéristiques et avantages
- Save time with a fast, highly sensitive substrate.
- Easily strip and reprobe membranes.
- Take multiple exposures of up to two days for each experiment.
- Reuse the substrate by filtering and storing in sodium azide.
Forme physique
25mM solution (11.6mg/ml), 100x concentrated, colorless solution.
Notes préparatoires
Storage conditions (working solution): Washing Buffer: 15 to 25 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
C S Martin et al.
BioTechniques, 18(5), 908-913 (1995-05-01)
Quantitative PCR and reverse transcription PCR (RT-PCR) are widely used in biomedical, industrial and other research applications to determine the number of RNA or DNA molecules of a specific type and/or sequence in a sample of interest. We have developed
Hoa Mai Nguyen et al.
Plant physiology, 163(2), 914-928 (2013-08-21)
The ω-3 polyunsaturated fatty acids account for more than 50% of total fatty acids in the green microalga Chlamydomonas reinhardtii, where they are present in both plastidic and extraplastidic membranes. In an effort to elucidate the lipid desaturation pathways in
Diane M Ramos et al.
BMC developmental biology, 6, 55-55 (2006-11-23)
Precise temporal and spatial regulation of transgene expression is a critical tool to investigate gene function in developing organisms. The most commonly used technique to achieve tight control of transgene expression, however, requires the use of specific DNA enhancers that
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique