API-RO
Roche
Alkaline Phosphatase (AP)
grade I, from calf intestine
Synonyme(s) :
AP, Alkaline Phosphatase
About This Item
Produits recommandés
Source biologique
bovine (calf) intestine
Niveau de qualité
Forme
suspension
Conditionnement
pkg of 1,500 U (10108138001)
pkg of 7,500 U (10108146001)
Fabricant/nom de marque
Roche
pH optimal
8.0-10.5
Absorption
7.6 at 278 nm (10 mg AP/ml)
Température de stockage
2-8°C
Description générale
Alkaline phosphatase catalyzes the removal of phosphate group from various compounds that are phosphorylated. It hydrolyzes 5′-monophosphate groups from both DNA and RNA. It can also hydrolyze 5′-diphosphate and 5′-triphosphate groups from RNA.
Spécificité
Denaturation is dependent on protein concentration - using less than 10 U/ml (0.1 mol/l glycine buffer, pH 10.5, 1 mmol/l MgCl2 and 0.1 mmol/l ZnCl2), AP is inactivated by a 10 minutes incubation at 65 °C.
Application
Qualité
Séquence
In some mammals (e.g., humans), there are at least 3 distinguishable isoenzymes: the intestinal, the placental and the form found in bone/liver/kidney.
Définition de l'unité
AP activity is not expressed in Armstrong units; a conversion factor for international units is not known.
Approx. 3.8 U (grade I AP) or 4.0 U (grade II AP) [+37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5].
Biochemica Information. 87 (grade I AP) :
1.0 U [+37 °C, QC assay conditions] = 0.79 U [+30 °C, QC assay conditions] = 0.57 U [+25 °C, QC assay conditions]
Forme physique
Notes préparatoires
- Activator: Divalent metal ions (Mg2+, Co2+, Mn2+)
- Amino alcohols (2-amino-2-methyl-1-propanol, diethanolamine)
Note that high concentrations of amino alcohols can as well act as competitive inhibitors (e.g., monoethanolamine in diethanolamine).
Storage conditions (working solution): The suspension is offered at a c= approx. 5 mg/ml.
If this suspension is diluted only 5 to 10-fold with further 3.2 M ammonium sulfate solution, 1 mM MgCl2, 0.1 mM ZnCl2, pH approx. 7, we would expect from general experience that a stability for some weeks might be given provided it is stored at 4 °C (no guarantee!).
Autres remarques
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
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Les clients ont également consulté
Protocoles
Alkaline Phosphatase (AP) Protocol
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