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CS0740

Sigma-Aldrich

Acid phosphatase Assay Kit

1 kit sufficient for 1,000 assays (multiwell plates), 1 kit sufficient for 100 assays (tubes)

Synonyme(s) :

Acid Phosphatase Enzyme Activity Kit

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About This Item

Code UNSPSC :
12161503
eCl@ss :
42010102
Nomenclature NACRES :
NA.84

Niveau de qualité

200

Utilisation

 kit sufficient for 1,000 assays (multiwell plates)
 kit sufficient for 100 assays (tubes)

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... ACP1(52) , ACP2(53)

Catégories apparentées

Application

The kit is used for the detection of acid phosphatase activity in tissues, whole cell extracts, column fractions and purified enzyme. The kit contains all the reagents required for a fast and simple acid phosphatase detection. The kit contains a standard solution and a control enzyme.

Actions biochimiques/physiologiques

Acid phosphatase is one of the acid hydrolases that normally reside in lysosomes. It is a marker for the identification of lysosomes in sub-cellular fractionations.

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Composants de kit seuls

Réf. du produit
Description
  • p-Nitrophenyl phosphate tablets 5 mg 20 tablets
  • Citrate buffer solution, 0.09 M pH 4.8 100 mL
  • p-Nitrophenol Standard solution 10 mM 1 mL
  • Acid phosphatase control enzyme .2 mL

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H351,H373

Classification des risques

Carc. 2 - STOT RE 2 Oral

Organes cibles

Liver,Kidney

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Yuan Li et al.
The Journal of cell biology, 215(2), 167-185 (2016-11-05)
Lysosomes degrade macromolecules and recycle metabolites as well as being involved in diverse processes that regulate cellular homeostasis. The lysosome is limited by a single phospholipid bilayer that forms a barrier to separate the potent luminal hydrolases from other cellular
Henning Staedt et al.
Head & face medicine, 16(1), 35-35 (2020-12-09)
The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membrane in vivo. Eight weeks after formation of a lateral full-thickness perforating bone defect in the
Cheng-Yen Lu et al.
Age (Dordrecht, Netherlands), 37(2), 33-33 (2015-04-13)
Ambient temperature reduction (ATR) can extend the lifespan of organisms, but the underlying mechanism is poorly understood. In this study, cellular degradation activity was evaluated in the muscle of an annual fish (Nothobranchius rachovii) reared under high (30 °C), moderate (25 °C)
Hua Zhao et al.
Journal of neuroscience methods, 217(1-2), 67-74 (2013-04-24)
Cobalamin (Cbl) utilization as a cofactor for methionine synthase and methylmalonyl-CoA mutase is dependent on the transport of Cbl through lysosomes and its subsequent delivery to the cytosol and mitochondria. We speculated that neuropathological conditions that impair lysosomal function (e.g.
Cameron G McCarthy et al.
American journal of physiology. Heart and circulatory physiology, 317(5), H1013-H1027 (2019-08-31)
Insufficient autophagy has been proposed as a mechanism of cellular aging, as this leads to the accumulation of dysfunctional macromolecules and organelles. Premature vascular aging occurs in hypertension. In fact, many factors that contribute to the deterioration of vascular function
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Articles

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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