11699105001

PCR Buffer Without MgCl₂, 10x concentrated

solution, pkg of 3 × 1 mL

• Synonyme(s) : buffer
• Code UNSPSC : 41105600

Propriétés

• Forme: solution
• Niveau de qualité: 100
• Conditionnement: pkg of 3 × 1 mL
• Fabricant/nom de marque: Roche
• Température de stockage: −20°C

Description

Description générale

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

Application

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).

Forme physique

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Autres remarques

For general laboratory use.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash