10109878001
Roche
Trypsin Inhibitor
from chicken egg white
Synonyme(s) :
trypisin
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About This Item
Code UNSPSC :
12352207
Produits recommandés
Source biologique
chicken egg white
Niveau de qualité
Forme
powder
Conditionnement
pkg of 1 g
Fabricant/nom de marque
Roche
Concentration
1 mg/mL
Technique(s)
cell culture | mammalian: suitable
pH optimal
3.8-4.5
Solubilité
water: soluble
Température de stockage
2-8°C
Description générale
Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).
Application
Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
- to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
- to neutralize trypsin post dissociation and trypsinization of brain tissues
- termination of tissue disaggregation and for subcultivation procedures
Notes préparatoires
Working concentration: 0
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.
Reconstitution
Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.
Stockage et stabilité
Store at 2 to 8 °C. (Store dry!)
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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